2018 Summer Intern Class

Congratulations to our 2018 Summer Interns Class!

Five college students joined DIEPS this summer to work on MISMS projects.

MISMS interns Allison Randy-Cofie, Garret Kern, Felix Wu, Anne Lheem, Josh Grubbs (front row, left to right)

Allison Randy-Cofie, Amherst College ’19

Project: Bioinformatics. Generating sub-sampled data sets of influenza A viruses from human, swine, equine, avian and canine hosts for all segments of the viral genome

Anne Lheem, Harvard University ’21

Project: Epidemiology. Identifying associations between influenza virus activity and antibiotic use on a global scale.

Felix Wu, Amherst College ’22

Project: Bibliometrics. Conducting a review of the publications produced by the MISMS and RAPIDD programs to assess their scientific impact and geographical diversity of co-authorship (published in Epidemics).

Garret Kern, Brown University ’22

Project: Bioinformatics. Creating a tool in Python to facilitate the downsampling of large genetic data sets by specified criteria (host, location, time, etc.). Creating R code to visualize pathogen data collected in humans and animal hosts (published in the Journal of Infectious Diseases)

Josh Grubs, Duke University ’18

Project: Biosafety. Creating protocols for chemical safety for use in Pakistan and other developing countries.

DOGS CAN BE A POTENTIAL RISK FOR FUTURE INFLUENZA PANDEMIC

MISMS researchers Martha Nelson and Nidia Trovao (Fogarty International Center) conducted an analysis of genetic sequence data obtained from dogs in Guangxi, China, published in mBio in June 2018. The study was conducted as part of NIAID’s Centers for Excellence in Influenza Research and Surveillance (CEIRS), in collaboration with Adolfo García-Sastre, who heads the Center for Research on Influenza Pathogenesis (CRIP) at the Icahn School of Medicine at Mount Sinai, New York City.

ASM Press Release

Washington, DC – June 5, 2018 – Dogs are a potential reservoir for a future influenza pandemic, according to a study published in the journal mBio. The study demonstrated that influenza virus can jump from pigs into canines and that influenza is becoming increasingly diverse in canines.

“The majority of pandemics have been associated with pigs as an intermediate host between avian viruses and human hosts. In this study, we identified influenza viruses jumping from pigs into dogs,” said study investigator Adolfo García-Sastre, PhD, director of the Global Health and Emerging Pathogens Institute and principal investigator, Center for Research on Influenza Pathogenesis (CRIP), Icahn School of Medicine at Mount Sinai, New York City.

Influenza can jump among animal reservoirs where many different strains are located; these reservoirs serve as mixing bowls for the genetic diversity of strains. Pandemic influenza occurs when viruses jump from animal reservoirs to humans; with no prior exposure to the virus, most people do not have immunity to these viruses. The main animal hosts for influenza are wild birds, poultry and other domestic birds in a species pack; swine; and horses. Some of the viral genes from the 2009 pandemic H1N1 virus originated in birds, from an avian virus that jumped to pigs, exchanged some of its genes with previously circulating swine viruses and then jumped from pigs into humans. Birds and swine are major reservoirs of viral genetic diversity, whereas equines and canines have historically been restricted to one or two stable influenza A viruses lineages with no or very limited transmission to humans.

Fifteen years ago, researchers documented an influenza virus in a horse jumping into a dog, and this created the first circulating canine influenza viruses. Five years ago, researchers identified an avian-origin H3N2 canine influenza virus circulating in farmed dogs in Guangdong, China.

“In our study, what we have found is another set of viruses that come from swine that are originally avian in origin, and now they are jumping into dogs and have been reassorted with other viruses in dogs. We now have H1N1, H3N2, and H3N8 in dogs. They are starting to interact with each other. This is very reminiscent of what happened in swine ten years before the H1N1 pandemic.”

Specifically, in the new study, the researchers sequenced the complete genomes of 16 influenza viruses obtained from canines in Southern China (Guangxi autonomous region) during 2013-2015. Other key study collaborators included Martha Nelson, PhD, a specialist in phylogenetic analysis and transmission reconstruction at CRIP, and Ying Chen, PhD, an influenza surveillance specialist who brought the samples from China. The researchers found that the genomes contained segments from three lineages that circulate in swine in China: North American triple reassortant H3N2, Eurasian avian-like H1N1, and pandemic H1N1. In addition, the swine-origin H1N1 viruses were transmitted onward in canines and reassorted with the CIV-H3N2 viruses that circulate endemically in Asian dogs, producing three novel reassortant CIV genotypes (H1N1r, /H1N2r, and H3N2r).

The viruses in the study were collected primarily from pet dogs presenting with respiratory symptoms at veterinary clinics. Dogs in certain regions of China, including Guangxi, are also raised for meat and street dogs roam freely, creating a more complex ecosystem for canine influenza virus transmission. “The new virus we have identified in our study is H1N1, but it comes from swine and is of avian origin, so it is different antigenically from the new H1N1s that were seen in the pandemic and a different origin as the previous H1N1 seen in humans,” said Dr. García-Sastre.

Future studies will focus on characterizing the virus further and assessing, using human sera, whether humans have existing immunity against canine H1N1 or not. “If there is a lot of immunity against these viruses, they will represent less of a risk, but we now have one more host in which influenza virus is starting to have a diverse genotypic and phenotypic characteristics, creating diversity in a host which is in very close contact to humans,” said Dr. García-Sastre. “The diversity in dogs has increased so much now that the type of combinations of viruses that can be created in dogs represent potential risk for a virus to jump to a dog into a human.”

The researchers say it is time to think about ways to restrict the circulation of the influenza virus in dogs. The United States is free of avian influenza because every time avian influenza has been detected in poultry in this country, the chickens or turkeys are culled and eliminated from circulation,” said Dr. García-Sastre. “There are attempts to restrict influenza virus in pigs through vaccination and one could consider vaccination for dogs.”

CRIP is one of five Centers of Excellence for Influenza Research and Surveillance funded by the National Institute of Allergy and Infectious Diseases.

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The American Society for Microbiology is the largest single life science society, composed of more than 30,000 scientists and health professionals. ASM’s mission is to promote and advance the microbial sciences.

ASM advances the microbial sciences through conferences, publications, certifications and educational opportunities. It enhances laboratory capacity around the globe through training and resources. It provides a network for scientists in academia, industry and clinical settings. Additionally, ASM promotes a deeper understanding of the microbial sciences to diverse audiences.

Post-doctoral opportunity

Post-doctoral fellowships in infectious disease modeling at the Fogarty International Center, NIH

The Division of International Epidemiology and Population Studies (DIEPS) of the Fogarty International Center (FIC), NIH, is seeking a post-doctoral fellow to work with Dr Cécile Viboud on a variety of infectious disease modeling projects. The projects fall under the auspices of two global computational modeling programs to control emerging and re-emerging infectious disease threats. The RAPIDD (Research and Policy for Infectious Disease Dynamics) program has established a global network of infectious disease modelers working at the research-policy interface, and provides evidence-based recommendations for disease control during outbreaks. The MISMS program leads research and training activities on the epidemiology and evolutionary dynamics of influenza viruses in humans and animal reservoirs. DIEPS has a long history of running computational projects and field studies, developing data-rich models, managing international collaborations and training programs, and translating research findings to policy.

The post-doc position would be supported with core FIC funds, allowing for independent, self-initiated research within broadly-defined areas of established DIEPS interests, including  the transmission dynamics of respiratory infections, the impact of new vaccines, and anti-microbial resistance. The candidate would also have the opportunity to collaborate with other DIEPS researchers studying infectious diseases, including Dr Martha Nelson‘s work on pathogen evolution at the human-animal interface. The candidate will also have the opportunity to be an instructor at international training workshops. Successful candidates will work in the historic Stone House on the NIH Bethesda campus (3 mi. from DC) and enjoy fruitful interactions with the dynamic community of NIH intramural scientists.

The successful candidate will have a doctoral degree (PhD or equivalent) in computational or evolutionary biology, applied statistics or physics, biostatistics, or related quantitative fields, with at least 1 year of research experience in computational modeling. Strong quantitative and communications skills as well as proficiency in analytical and dynamic modeling, and/or phylogenetic analysis is required. The ability to critically evaluate data, publish scientific papers, work in interdisciplinary environments, and present at conferences is essential.

Interested candidates should contact Cécile Viboud (viboudc@mail.nih.gov). Applications should include a cover letter, a CV, a brief statement of research interests, and the names (and contact info) of three references. Salary will be commensurate with experience and NIH guidelines.

Lawton Chiles International ‘Stone’ House

MISMS faculty teach 5-day training workshop in Dubai, UAE

MISMS workshop participants in Dubai, UAE

From February 5-9, 2018, MISMS staff taught a hands-on workshop in Dubai, UAE for participants coming from multiple countries in South Asia, including India, Pakistan, Nepal, and Bangladesh. The course included time-series analysis, R, disease modeling, phylogenetic analysis, and opportunities for participants to study their own data.

Many of the participants were part of the FIC-led MAL-ED study (including sites in India, Nepal, and Bangladesh) and Pakistan-based BEP study.